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classical monocyte isolation kit  (Miltenyi Biotec)


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    Miltenyi Biotec classical monocyte isolation kit
    Classical Monocyte Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/classical monocyte isolation kit/product/Miltenyi Biotec
    Average 97 stars, based on 144 article reviews
    classical monocyte isolation kit - by Bioz Stars, 2026-03
    97/100 stars

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    classical monocyte isolation kit  (Miltenyi Biotec)
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    human classical monocyte isolation kit  (Miltenyi Biotec)
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    Miltenyi Biotec human classical monocyte isolation kit
    Induction of <t>human</t> osteoclast progenitors under various culture conditions. (A) Morphological features of human <t>classical</t> <t>monocytes</t> cultured under the following four conditions: (i) M-CSF (10 ng/mL), (ii) G-M-CSF (50 ng/mL), (iii) GM-CSF + IL-4 (50 ng/mL each), and (iv) GM-CSF (50 ng/mL) + tumor necrosis factor-alpha (TNF-α; 10 ng/mL). Scale bar: 100 μm. (B) qRT-PCR analysis of the four cell types for the expression of dendritic cell markers HLA-DRA1 and CD80 . Data are representative of three independent experiments.
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    Induction of human osteoclast progenitors under various culture conditions. (A) Morphological features of human classical monocytes cultured under the following four conditions: (i) M-CSF (10 ng/mL), (ii) G-M-CSF (50 ng/mL), (iii) GM-CSF + IL-4 (50 ng/mL each), and (iv) GM-CSF (50 ng/mL) + tumor necrosis factor-alpha (TNF-α; 10 ng/mL). Scale bar: 100 μm. (B) qRT-PCR analysis of the four cell types for the expression of dendritic cell markers HLA-DRA1 and CD80 . Data are representative of three independent experiments.

    Journal: Bone Reports

    Article Title: Distinct osteoclastogenic potential of granulocyte-macrophage colony-stimulating factor-induced monocyte subsets

    doi: 10.1016/j.bonr.2025.101886

    Figure Lengend Snippet: Induction of human osteoclast progenitors under various culture conditions. (A) Morphological features of human classical monocytes cultured under the following four conditions: (i) M-CSF (10 ng/mL), (ii) G-M-CSF (50 ng/mL), (iii) GM-CSF + IL-4 (50 ng/mL each), and (iv) GM-CSF (50 ng/mL) + tumor necrosis factor-alpha (TNF-α; 10 ng/mL). Scale bar: 100 μm. (B) qRT-PCR analysis of the four cell types for the expression of dendritic cell markers HLA-DRA1 and CD80 . Data are representative of three independent experiments.

    Article Snippet: Classical monocytes were isolated from the PBMCs of healthy volunteers using the AutoMACS cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany) and Human Classical Monocyte Isolation Kit (Miltenyi Biotec), according to the manufacturer's instructions.

    Techniques: Cell Culture, Quantitative RT-PCR, Expressing